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pGL-FLKif3B
(Plasmid #13743)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13743 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGREENLANTERN-1
  • Backbone size w/o insert (bp) 4996
  • Vector type
    Mammalian Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse Kif3B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Entrez Gene
    Kif3b (a.k.a. RP23-192G18.6, AI854312, AW549267, mKIAA0359)
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL-FLKif3B was a gift from Linda Wordeman (Addgene plasmid # 13743 ; http://n2t.net/addgene:13743 ; RRID:Addgene_13743)
  • For your References section:

    Expression and partial characterization of kinesin-related proteins in differentiating and adult skeletal muscle. Ginkel LM, Wordeman L. Mol Biol Cell. 2000 Dec . 11(12):4143-58. 10.1091/mbc.11.12.4143 PubMed 11102514