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pUF-Cas9-pre-sgRNA
(Plasmid #137845)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 137845 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUF-1
  • Vector type
    CRISPR
  • Selectable markers
    yDHODH

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jinek et al, Addgene #42234

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUF-Cas9-pre-sgRNA was a gift from Josh Beck (Addgene plasmid # 137845 ; http://n2t.net/addgene:137845 ; RRID:Addgene_137845)
  • For your References section:

    EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole. Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, Goldberg DE, Beck JR. Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168. 10.1111/cmi.13168 PubMed 31990132