pVAX1-BMP7
(Plasmid
#137910)
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PurposeConstitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNA
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Depositing Lab
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Depositing OrganizationLudwig Boltzmann Institute for Traumatology Research Centre in Cooperation with AUVA
Located in Austria -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 137910-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 137910-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepVAX1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBMP7
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SpeciesH. sapiens (human)
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Entrez GeneBMP7 (a.k.a. OP-1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 137910-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 137910-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVAX1-BMP7 was a gift from Georg Feichtinger (Addgene plasmid # 137910 ; http://n2t.net/addgene:137910 ; RRID:Addgene_137910) -
For your References section:
Constitutive and inducible co-expression systems for non-viral osteoinductive gene therapy. Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, van Griensven M, Redl H. Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184. PubMed 24554273
