Pu Lab - Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
(Pooled Library #138015)
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Purpose
This AAV CRISPR knockout library is designed to investigate cardiac gene regulation.
Guide RNAs target transcriptional regulators expressed in mouse cardiomyocytes and a subset of GATA4 regulated genes. -
Vector Backbone
AAV-U6-gRNA-TnT-Cre - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 138015 | gRNA pooled library† | 1 | $ 380 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item. The AAV backbone expresses a Cre-recombinase and can be used with Cre-activatable Cas9.
Library Details
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SpeciesMouse
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Genes targeted2,444
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gRNAs14,671
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Controls7 gRNAs target human genome
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration25 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library
Please visit https://www.biorxiv.org/content/10.1101/808402v1 for bioRxiv preprint.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library was a gift from William Pu (Addgene #138015) -
For your References section:
In vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation. VanDusen N.J., Lee J.Y., Gu W., Sethi I., Zheng Y., King J.S., Zhou P., Suo S., Guo Y., Ma Q., Yuan G., Pu W.T. bioRxiv 808402v1 https://www.biorxiv.org/content/10.1101/808402v1.full.