Purpose(Empty Backbone) Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138096||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typePlant Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Tetracycline, 10 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYPQ131-STU-Lb was a gift from Yiping Qi (Addgene plasmid # 138096 ; http://n2t.net/addgene:138096 ; RRID:Addgene_138096)
For your References section:Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems. Zhang Y, Ren Q, Tang X, Liu S, Malzahn AA, Zhou J, Wang J, Yin D, Pan C, Yuan M, Huang L, Yang H, Zhao Y, Fang Q, Zheng X, Tian L, Cheng Y, Le Y, McCoy B, Franklin L, Selengut JD, Mount SM, Que Q, Zhang Y, Qi Y. Nat Commun. 2021 Mar 29;12(1):1944. doi: 10.1038/s41467-021-22330-w. 10.1038/s41467-021-22330-w PubMed 33782402