pT7NT*-Bri2 113-231 R221E
(Plasmid #138134)

• Purpose: Expresseds human Bri2 BRICHOS R221E mutant in E. coli
• Depositing Lab: Jan Johansson
• Publication: Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 10.1038/s42003-020-0757-z.

Backbone

• Vector backbone: modified pET vector
• Backbone size w/o insert (bp): 5647
• Total vector size (bp): 6004
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Bri2 BRICHOS R221E
• Species: H. sapiens (human)
• Insert Size (bp): 357
• Entrez Gene: ITM2B (a.k.a. ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, RDGCA, imBRI2)
• Promoter: T7
• Tag / Fusion Protein:
  • NT* tag derived from spider silk proteins (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminator primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry