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(Plasmid #138209)


Item Catalog # Description Quantity Price (USD)
Plasmid 138209 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 5500
  • Vector type
    Mammalian Expression ; T7 Transcription
  • Promoter CMV Promoter, T7 Promoter
  • Tag / Fusion Protein
    • TAG sequence

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An RNA of interest can be cloned into this vector in order to append a 25-nucleotide TGT recognition element to its 3' end. The TGT recognition element, or TAG, has short spacer arms at either side.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-(empty)-TAG was a gift from Neal Devaraj (Addgene plasmid # 138209 ; ; RRID:Addgene_138209)
  • For your References section:

    Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG). Busby KN, Devaraj NK. Methods Enzymol. 2020;641:373-399. doi: 10.1016/bs.mie.2020.03.009. Epub 2020 Apr 20. 10.1016/bs.mie.2020.03.009 PubMed 32713531