PurposeBooster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138375||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 7096
Vector typeMammalian Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast), Synthetic
Insert Size (bp)2600
- Promoter CAG
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAGGS-4511NES was a gift from Michiyuki Matsuda (Addgene plasmid # 138375 ; http://n2t.net/addgene:138375 ; RRID:Addgene_138375)
For your References section:Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. Watabe T, Terai K, Sumiyama K, Matsuda M. ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941. 10.1021/acssensors.9b01941 PubMed 32101394