pCAGGS-4493NES
(Plasmid
#138373)
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PurposeBooster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 7327
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBooster-PKA
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)2600
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-4493NES was a gift from Michiyuki Matsuda (Addgene plasmid # 138373 ; http://n2t.net/addgene:138373 ; RRID:Addgene_138373) -
For your References section:
Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. Watabe T, Terai K, Sumiyama K, Matsuda M. ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941. 10.1021/acssensors.9b01941 PubMed 32101394
