pCAGGS-4493NES
(Plasmid #138373)

• Purpose: Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.
• Depositing Lab: Michiyuki Matsuda
• Publication: Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.

Backbone

• Vector backbone: pCAGGS
  (Search Vector Database)
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7327
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Booster-PKA
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 2600
• Promoter: CAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pCAGGS-5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.