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PurposeExpress EGFP with an upstream T7 promoter in pUC19 backbone
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Depositing Lab
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Depositing OrganizationBroad Institute
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 138586-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 138586-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 3844
- Total vector size (bp) 4564
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP
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Insert Size (bp)720
Resource Information
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A portion of this plasmid was derived from a plasmid made byEGFP was cloned from pcDNA3.1(+)IRES GFP, which was obtained from Kathleen_L Collins (Addgene plasmid # 51406.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the depositor sequence linked in the supplemental documents section is different compared to the Addgene verified sequence. We recommend viewing the Addgene verified sequence.
DNA (Catalog # 138586-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 138586-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19 - T7 pro - IRES - EGFP was a gift from Fei Chen (Addgene plasmid # 138586 ; http://n2t.net/addgene:138586 ; RRID:Addgene_138586) -
For your References section:
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8. 10.1038/s41587-019-0331-8 PubMed 31844291
Map uploaded by the depositor.
