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(Plasmid #138958)


Item Catalog # Description Quantity Price (USD)
Plasmid 138958 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8489
  • Vector type
    Insect Expression ; UAS transgene vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    chitin synthase
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
  • Mutation
    8 bp that are in the region that encodes the catalytic domain are mutated to encode the mosquito sequence.
  • GenBank ID
  • Entrez Gene
  • Promoter UAS
  • Tag / Fusion Protein
    • Neon Green fused to C terminus of Kkv

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer CTGGGTGCTGCCTGCGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I obtained the Neon Green DNA from Allele Biotech and the kkv cDNA from the fly genome project.

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-kkv-mos::NG was a gift from Paul Adler (Addgene plasmid # 138958 ; ; RRID:Addgene_138958)
  • For your References section:

    The localization of chitin synthase mediates the patterned deposition of chitin in developing Drosophila bristles. Adler PN. bioRxiv 2020 10.1101/718841