RCAS(A) mWnt5a (CT#539)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13936||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneRCASBP 2A
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneWnt5a (a.k.a. 8030457G12Rik, Wnt-5a)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
Full-legth ORF of mWnt-5a;
5’ NcoI-primer : CATGACCATG GCCATGAAGC CCATTGGAAT ATTAA
3’BamHI-primer: CGGGATCCTA TTTGCACACG AACTGATCC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS(A) mWnt5a (CT#539) was a gift from Cliff Tabin (Addgene plasmid # 13936 ; http://n2t.net/addgene:13936 ; RRID:Addgene_13936)
For your References section:Dual roles of Wnt signaling during chondrogenesis in the chicken limb. Hartmann C, Tabin CJ. Development. 2000 Jul . 127(14):3141-59. PubMed 10862751