RCAS (A) Wnt3a (CT#169)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13976||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11600
Vector typeRetroviral ; avian expression
Growth in Bacteria
Copy numberLow Copy
SpeciesM. musculus (mouse)
Entrez GeneWnt3a (a.k.a. Wnt-3a, vt)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
A mouse Wnt3a cDNA encoding the entire open reading frame was cloned into the shuttle vector SLAX-13 and then subloned into the RCAS retroviral vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RCAS (A) Wnt3a (CT#169) was a gift from Cliff Tabin (Addgene plasmid # 13976 ; http://n2t.net/addgene:13976 ; RRID:Addgene_13976)
For your References section:Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud. Kengaku M, Capdevila J, Rodriguez-Esteban C, De La Pena J, Johnson RL, Belmonte JC, Tabin CJ. Science. 1998 May 22. 280(5367):1274-7. 10.1126/science.280.5367.1274 PubMed 9596583