Scarlet I-FRET 10-NeonGreen
(Plasmid
#139364)
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PurposeFRET Standard
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Depositing Lab
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Depositing OrganizationIndiana University School of Medicine, Indianapolis
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139364 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 139364-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 139364-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScarlet 10AA Neon green
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Mfe1 (unknown if destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer CTC TAC AAA TGT GGT ATG G
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
we used #98839 (mScarlet-I-mTurquoise2) as a base for the vector and replaced Turquoise2 with Neon green.
DNA (Catalog # 139364-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 139364-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Scarlet I-FRET 10-NeonGreen was a gift from Richard Day (Addgene plasmid # 139364 ; http://n2t.net/addgene:139364 ; RRID:Addgene_139364) -
For your References section:
PIE-FLIM Measurements of Two Different FRET-Based Biosensor Activities in the Same Living Cells. Reissaus CA, Day KH, Mirmira RG, Dunn KW, Pavalko FM, Day RN. Biophys J. 2020 Apr 21;118(8):1820-1829. doi: 10.1016/j.bpj.2020.03.003. Epub 2020 Mar 10. 10.1016/j.bpj.2020.03.003 PubMed 32191861
