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pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
(Plasmid #139482)


Item Catalog # Description Quantity Price (USD)
Plasmid 139482 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 9668
  • Total vector size (bp) 12323
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Alt name
    phytochrome b
  • Species
    A. thaliana (mustard weed)
  • Mutation
    G229L and deletion of aa Y at position 235 in mcherry
  • Entrez Gene
    PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
  • Promoter Tet responsible element
  • Tag / Fusion Protein
    • mCherry-HRasCT (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer not determined
  • 3′ sequencing primer GGGCCCTCACATTGCCAAA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Depositor confirmed that mutation G229L and deletion of amino acid Y at position 235 in mCherry has no functional effect.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIneo-TRE-hPhyB621- mCherry-HRasCT was a gift from Kazuhiro Aoki (Addgene plasmid # 139482 ; ; RRID:Addgene_139482)