pCSIIbleo-PIF3-mEGFP
(Plasmid #139483)

• Purpose: Expresses PIF3-mEGFP in mammalian cells.
• Depositing Lab: Kazuhiro Aoki
• Publication: Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9.

Backbone

• Vector backbone: pCSIIbleo
• Backbone size w/o insert (bp): 10144
• Total vector size (bp): 11194
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PIF3
• Alt name: Phytochrome interacting factor 3
• Species: A. thaliana (mustard weed)
• Mutation: PIF3 1-100 aa
• Entrez Gene: PIF3 (a.k.a. AT1G09530, F14J9.19, F14J9_19, PAP3, PHOTOCURRENT 1, PHYTOCHROME INTERACTING FACTOR 3, PHYTOCHROME-ASSOCIATED PROTEIN 3, POC1, phytochrome interacting factor 3, purple acid phosphatase 3)
• Promoter: Human EF1a promoter
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: not determined
• 3′ sequencing primer: GGGCCCTCACATTGCCAAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry