MEN2A-MAC
(Plasmid
#139634)
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PurposeMAC-tagged gene expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerMarkku Varjosalo, Addgene plasmid #108077
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMEN2A
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SpeciesH. sapiens (human)
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MutationC634W
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Entrez GeneRET (a.k.a. CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1)
- Promoter CMV
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Tag
/ Fusion Protein
- StrepIII-HA-BirA* (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MEN2A-MAC was a gift from Markku Varjosalo (Addgene plasmid # 139634 ; http://n2t.net/addgene:139634 ; RRID:Addgene_139634) -
For your References section:
PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and Inhibits the RET Mutant MEN2A Oncogenic Potential. Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, Saarma M, Varjosalo M. iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871. Epub 2020 Jan 31. 10.1016/j.isci.2020.100871 PubMed 32062451
