pLVX Nup62Fv2GFP
(Plasmid #139683)

• Purpose: Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter
• Depositing Lab: Edward Campbell
• Publication: Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/s41564-020-0735-8. doi: 10.1038/s41564-020-0735-8.

Backbone

• Vector backbone: pLVX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 11455
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nup62
• Species: H. sapiens (human)
• Insert Size (bp): 3342
• GenBank ID: BC003663.2 23636
• Entrez Gene: NUP62 (a.k.a. IBSN, SNDI, p62)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Dimerization domain FKBP (C terminal on insert)
  • 2 copies of GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BstB1 (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: PGK rev

Resource Information

• Supplemental Documents:
  • pLVX hNUP62Fv2GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry