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dRED
(Plasmid #139836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139836 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 139836-D50 50 μg of DNA in Tris buffer $495
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DNA 139836-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Life Technologies/Thermo Fisher
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 10922
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dRNase H1-EGFP-dCas9
  • Alt name
    deadRNase H1-EGFP-deadCas9
  • Species
    dRNase H1 (H. sapiens), dCas9 (S. pyogenes)
  • Insert Size (bp)
    5844
  • Mutation
    RNase H1 protein contains D210N mutation. dCas9 protien contains D10A and H840A mutations.
  • GenBank ID
    RNase H1 (BT019670.1) Cas9 (AAK33936.1)
  • Entrez Gene
    RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
  • Promoter tet-inducible CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dRED was a gift from Karim Mekhail (Addgene plasmid # 139836 ; http://n2t.net/addgene:139836 ; RRID:Addgene_139836)

  • For your References section:

    Nucleolar RNA polymerase II drives ribosome biogenesis. Abraham KJ, Khosraviani N, Chan JNY, Gorthi A, Samman A, Zhao DY, Wang M, Bokros M, Vidya E, Ostrowski LA, Oshidari R, Pietrobon V, Patel PS, Algouneh A, Singhania R, Liu Y, Yerlici VT, De Carvalho DD, Ohh M, Dickson BC, Hakem R, Greenblatt JF, Lee S, Bishop AJR, Mekhail K. Nature. 2020 Sep;585(7824):298-302. doi: 10.1038/s41586-020-2497-0. Epub 2020 Jul 15. 10.1038/s41586-020-2497-0 PubMed 32669707
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