EPHB4-2A-EGFP
(Plasmid
#139968)
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PurposeDonor vector to knockin EGFP (separated by 2A) into EPHB4 allele
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Depositing Lab
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Depositing OrganizationMorgridge Institute for Research
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 139968-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 139968-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePCR2.1
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman EPHB4 knockin homology arms
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SpeciesH. sapiens (human)
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Entrez GeneEPHB4 (a.k.a. CMAVM2, HFASD, HTK, LMPHM7, MYK1, TYRO11)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 139968-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 139968-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EPHB4-2A-EGFP was a gift from James Thomson (Addgene plasmid # 139968 ; http://n2t.net/addgene:139968 ; RRID:Addgene_139968) -
For your References section:
Functional characterization of human pluripotent stem cell-derived arterial endothelial cells. Zhang J, Chu LF, Hou Z, Schwartz MP, Hacker T, Vickerman V, Swanson S, Leng N, Nguyen BK, Elwell A, Bolin J, Brown ME, Stewart R, Burlingham WJ, Murphy WL, Thomson JA. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6072-E6078. doi: 10.1073/pnas.1702295114. Epub 2017 Jul 10. 10.1073/pnas.1702295114 PubMed 28696312
Map uploaded by the depositor.
