pBS cIhh (CT#431)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13997||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBS SK-
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
Insert Size (bp)1680
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Total coding sequence plus 327bp 5’ UTR and 130bp 3’ UTR, from Andrea Vortkamp.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS cIhh (CT#431) was a gift from Cliff Tabin (Addgene plasmid # 13997 ; http://n2t.net/addgene:13997 ; RRID:Addgene_13997)
For your References section:Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein. Vortkamp A, Lee K, Lanske B, Segre GV, Kronenberg HM, Tabin CJ. Science. 1996 Aug 2. 273(5275):613-22. 10.1126/science.273.5275.613 PubMed 8662546