pGEM cOp (CT#625)
(Plasmid #13941)

• Depositing Lab: Cliff Tabin
• Publication: Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Op
• Alt name: Osteopontin
• Species: G. gallus (chicken)
• Insert Size (bp): 645
• Mutation: Amino acids 34-260, V98M, G216D, R235A*
• Entrez Gene: SPP1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

645 bp RT-PCR product corresponding to AA 34-260 of sequenced published by Castagnola et al., 1991.

*Note that these mutations were not reported but were found during Addgene's quality control sequencing. They are not known to affect function and may only be polymorphisms.