pAS_4xMma PylT_GFP 150TAG 6xHis
(Plasmid #140015)

• Purpose: Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.

Backbone

• Vector backbone: pAS
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfGFP
• Alt name: 4xh7SK-Mma PylT cassette
• Species: Aequorea victoria
• Insert Size (bp): 750
• Mutation: 150TAG
• Promoter: EF1
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pas_4xmmapylt_sfgfp150tag-6xhis

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry