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Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.
Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140009pAS_4xMma PylT_FLAG-Mma PylRSPlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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140010pAS_4xMma PylT(UUA)_FLAG-AcKRSPlasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration
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140011pAS_4xMx PylT_FLAG-Mx PylRSPlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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140012pAS_4xMx PylT_FLAG-Mx PylRS YAPlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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140013pAS_4xMx PylT C41CA_FLAG_Mx PylRSPlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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140014pAS_4xMx PylT C41CA_FLAG_Mx PylRS YAPlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
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140015pAS_4xMma PylT_GFP 150TAG 6xHisPlasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration
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140016pAS_4xMx PylT_GFP 150TAGPlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration
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140017pAS_4xMx PylT C41CA_GFP 150TAGPlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration
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154762pAS_FLAG-Mma PylRSplasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration
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154764pAS_FLAG-Mx1201 PylRSplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration
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154765pAS_sfGPF 150TAGplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
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154766pAS_4xMma PylT sfGPF 150TAGplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
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154782pUC_h7SK Mma PylT (CUA)plasmid with single copy of Mma PylT (CUA anticodon)
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Recombinant Antibodies from Article