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Addgene

pUC_h7SK Mma PylT (CUA)
(Plasmid #154782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Backbone manufacturer
    IDT
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mma PylT
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    312
  • Mutation
    U25C
  • Promoter h7SK

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC_h7SK Mma PylT (CUA) was a gift from Simon Elsaesser (Addgene plasmid # 154782 ; http://n2t.net/addgene:154782 ; RRID:Addgene_154782)

  • For your References section:

    Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells. Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ. ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. 10.1021/acschembio.8b00571 PubMed 30260624
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