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pCDF_LuxR
(Plasmid #140039)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140039 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDF
  • Total vector size (bp) 3379
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pLuxL and LuxR
  • Species
    Synthetic
  • Insert Size (bp)
    923

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer NNNNggtaccACCTGTACGATCCTACAGGT
  • 3′ sequencing primer CTGTCCGTACTTCAAAAATTAATAAGGATCCNNNN
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF_LuxR was a gift from Yolanda Schaerli (Addgene plasmid # 140039 ; http://n2t.net/addgene:140039 ; RRID:Addgene_140039)
  • For your References section:

    Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch. Barbier I, Perez-Carrasco R, Schaerli Y. Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361. 10.15252/msb.20199361 PubMed 32529808