pCDF_LuxR
(Plasmid #140039)

• Purpose: Constutitive expression of LuxR protein.
• Depositing Lab: Yolanda Schaerli
• Publication: Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361.

Backbone

• Vector backbone: pCDF
• Total vector size (bp): 3379
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 50 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pLuxL and LuxR
• Species: Synthetic
• Insert Size (bp): 923

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: NNNNggtaccACCTGTACGATCCTACAGGT
• 3′ sequencing primer: CTGTCCGTACTTCAAAAATTAATAAGGATCCNNNN

Resource Information

• Supplemental Documents:
  • pcdf_luxR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.