PurposeConstutitive expression of LuxR protein.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140039||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3379
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin and Streptomycin
Copy numberLow Copy
Gene/Insert namepLuxL and LuxR
Insert Size (bp)923
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer NNNNggtaccACCTGTACGATCCTACAGGT
- 3′ sequencing primer CTGTCCGTACTTCAAAAATTAATAAGGATCCNNNN (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/10.1101/849711v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDF_LuxR was a gift from Yolanda Schaerli (Addgene plasmid # 140039 ; http://n2t.net/addgene:140039 ; RRID:Addgene_140039)
For your References section:Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch. Barbier I, Perez-Carrasco R, Schaerli Y. Mol Syst Biol. 2020 Jun;16(6):e9361. doi: 10.15252/msb.20199361. 10.15252/msb.20199361 PubMed 32529808