Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #140198)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140198 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    DOI: 10.1039/C8SC02870B
  • Backbone size w/o insert (bp) 14777
  • Total vector size (bp) 25045
  • Modifications to backbone
    We added Leu Kan markers for more stringent selection during cloning.
  • Vector type
    CRISPR, Synthetic Biology ; Expression in Aspergillus nidulans and related filamentous fungi.
  • Selectable markers
    LEU2, URA3 ; Af riboB

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
  • Species
    Synthetic; Lachnospiraceae bacterium
  • Insert Size (bp)
  • Mutation
    D832A DNase deactivated, D156R for improved activity at 25 °C
  • Promoter PgpdA
  • Tag / Fusion Protein
    • VPR (VP64-p65-Rta), NLS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC was a gift from Yit Heng Chooi (Addgene plasmid # 140198 ; ; RRID:Addgene_140198)
  • For your References section:

    CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi. Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH. ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197. 10.1021/acssynbio.0c00197 PubMed 32526136