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pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
(Plasmid #140202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140202 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 140202-D50 50 μg of DNA in Tris buffer $495
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DNA 140202-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pKW20088
  • Backbone manufacturer
    doi:10.1038/nchembio.1366
  • Backbone size w/o insert (bp) 14245
  • Total vector size (bp) 15879
  • Vector type
    CRISPR, Synthetic Biology ; Proof-of-concept test target of a in filamentous fungi.
  • Selectable markers
    URA3 ; Af pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    709
  • Mutation
    G174D
  • Promoter PelcA

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PelcA-mCherry has no observable basal expression in Aspergillus nidulans, constituting a good test target for CRISPRa. Also contains PacI NotI sites to clone other components, and allows cloning in Saccharomyces cerevisiae. The mCherry mutation G174D does not appear to affect fluorescence.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI was a gift from Yit Heng Chooi (Addgene plasmid # 140202 ; http://n2t.net/addgene:140202 ; RRID:Addgene_140202)

  • For your References section:

    CRISPR-mediated activation of biosynthetic gene clusters for bioactive molecule discovery in filamentous fungi. Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH. ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197. 10.1021/acssynbio.0c00197 PubMed 32526136
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