|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140594||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6456
Modifications to backboneNourseothricin (NatMX6) resistance instead of URA3 selection marker
Vector typeYeast Expression
Selectable markersNAT (Nourseothricin)
Growth in Bacteria
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCplac33-NAT was a gift from David Sabatini (Addgene plasmid # 140594 ; http://n2t.net/addgene:140594 ; RRID:Addgene_140594)
For your References section:MCART1/SLC25A51 is required for mitochondrial NAD transport. Kory N, uit de Bos J, van der Rijt S, Jankovic N, Güra M, Arp N, Pena IA, Prakash G, Chan SH, Kunchok T, Lewis, CA, Sabatini DM. Science Advances 09 Sep 2020: eabe5310 10.1126/sciadv.abe5310