PurposedCas9 & Csy4
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140664||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namedCas9 & Csy4
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
GenBank file with original annotations can be downloaded from the "Resource Information" section.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJ1996_v2 was a gift from Yolanda Schaerli (Addgene plasmid # 140664 ; http://n2t.net/addgene:140664 ; RRID:Addgene_140664)
For your References section:Multistable and dynamic CRISPRi-based synthetic circuits. Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y. Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. 10.1038/s41467-020-16574-1 PubMed 32488086