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Multistable and dynamic CRISPRi-based synthetic circuits.
Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y
Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140664pJ1996_v2dCas9 & Csy4
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140665pJ2018dCas9, LuxR & Csy4
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140666pJ2077.2Controller plasmid for TS circuit and cL and cR controls
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140667pJ2076.2_TSToggle switch (TS)
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140668pJ2076.2_cLcL control for toggle switch experiment
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140669pJ2076.2_cRcR control for toggle switch experiment
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140670pJ2042.23-color stripe
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140671pJ2042.2_Bs1-color stripe (“BLUE”)
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140672pJ2048.2_Gs1-color stripe (“GREEN”)
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140673pJ2048_2xNOTDouble-inverter
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140674pJ2072.2_CRISPRlatorCRISPRlator
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140675pJ2072.2_c1CRISPRlator control (“open ring”)
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140676pJ2044NOT gate with sg-1
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140677pJ2043NOT gate with sg-2
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140678pJ2039NOT gate with sg-3
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140679pJ2040NOT gate with sg-4
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140680pJ2042.2_BroccBroccoli Stripe
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140681pJ2043_N2onlyControl (“C”) lacking sg-2
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140682pJ2039_N2onlyControl (“C”) lacking sg-3
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140683pJ2040_N2onlyControl (“C”) lacking sg-4
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140684pJ2044_t4NOT gate with sg-1t4
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140685pJ2040_t4NOT gate with sg-4t4
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140686pJ2042.2_GFPonly1-color stripe
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140687pJ2048.23-color stripe (different sgRNAs)
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140688pJ2042.2_invRep3-color stripe (inverted reporters)
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140689pJ2044_N2onlyControl (“C”) lacking sg-1
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