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pJ2043_N2only
(Plasmid #140681)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140681 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Control (“C”) lacking sg-2

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ2043_N2only was a gift from Yolanda Schaerli (Addgene plasmid # 140681 ; http://n2t.net/addgene:140681 ; RRID:Addgene_140681)
  • For your References section:

    Multistable and dynamic CRISPRi-based synthetic circuits. Santos-Moreno J, Tasiudi E, Stelling J, Schaerli Y. Nat Commun. 2020 Jun 2;11(1):2746. doi: 10.1038/s41467-020-16574-1. 10.1038/s41467-020-16574-1 PubMed 32488086