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pPB-tetpA-hNEUROG2-iRPT
(Plasmid #140764)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140764 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 140764-D50 50 μg of DNA in Tris buffer $495
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DNA 140764-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pPB-MCSfw-pA
  • Total vector size (bp) 9558
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    iRFP713
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    948

Gene/Insert 2

  • Gene/Insert name
    PuroR
  • Alt name
    Puromycin N-acetyl transferase
  • Species
    Streptomyces alboniger
  • Insert Size (bp)
    597

Gene/Insert 3

  • Gene/Insert name
    rtTAM2
  • Insert Size (bp)
    747

Gene/Insert 4

  • Gene/Insert name
    NEUROG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    819
  • GenBank ID
    NM_024019.4
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-tetpA-hNEUROG2-iRPT was a gift from Hideyuki Okano (Addgene plasmid # 140764 ; http://n2t.net/addgene:140764 ; RRID:Addgene_140764)

  • For your References section:

    Evaluating the efficacy of small molecules for neural differentiation of common marmoset ESCs and iPSCs. Yoshimatsu S, Nakamura M, Nakajima M, Nemoto A, Sato T, Sasaki E, Shiozawa S, Okano H. Neurosci Res. 2019 Oct 3. pii: S0168-0102(19)30262-7. doi: 10.1016/j.neures.2019.09.005. 10.1016/j.neures.2019.09.005 PubMed 31586586
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