pLenti-lox-GFP shRNA p19-2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14091||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable from Addgene (#11795)
- Backbone size w/o insert (bp) 8000
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namep19 ARF shRNA -2
SpeciesM. musculus (mouse)
Insert Size (bp)50
Entrez GeneCdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer H1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-lox-GFP shRNA p19-2 was a gift from Tyler Jacks (Addgene plasmid # 14091 ; http://n2t.net/addgene:14091 ; RRID:Addgene_14091)
For your References section:Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Sage J, Miller AL, Perez-Mancera PA, Wysocki JM, Jacks T. Nature. 2003 Jul 10. 424(6945):223-8. 10.1038/nature01764 PubMed 12853964