pLX307/PTPN11 T73I
(Plasmid #140939)

• Purpose: expresses PTPN11 T73I
• Depositing Lab: Kimberly Stegmaier
• Publication: Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. doi: 10.1158/2159-8290.CD-19-0209. Epub 2019 Nov 26.

Backbone

• Vector backbone: pLX307
• Backbone size w/o insert (bp): 10065
• Total vector size (bp): 10500
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ]
• Alt name: CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Mutation: T73I
• GenBank ID: NM_002834
• Entrez Gene: PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
• Promoter: EF1a
• Tag / Fusion Protein:
  • V5

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a_F
• 3′ sequencing primer: WPRE

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry