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pDESTFLAGHApuro3-Ago2K533AK570A
(Plasmid #14104)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 14104 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDESTFLAGHApuro3
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ago2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2562
  • Mutation
    changed Lys533 to Ala, Lys570 to Alanine
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LINC00980, PPD, Q10)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site gateway (not destroyed)
  • 3′ cloning site gateway (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTFLAGHApuro3-Ago2K533AK570A was a gift from Thomas Tuschl (Addgene plasmid # 14104 ; http://n2t.net/addgene:14104 ; RRID:Addgene_14104)
  • For your References section:

    Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein. Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ. Nature. 2005 Mar 31. 434(7033):666-70. 10.1038/nature03514 PubMed 15800629