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pCS2+ Grx1-roCherry
(Plasmid #141068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141068 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 141068-D50 50 μg of DNA in Tris buffer $495
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DNA 141068-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4072
  • Total vector size (bp) 5149
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grx1-roCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1077
  • Mutation
    Changed Alanine 150 to Cysteine, inserted Threonine after Cysteine 150, changed Serine 151 to Glutamate, changed Lysine 203 to Cysteine
  • Promoter CMV, SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TCGGAGCAAGCTTGATTTAGGTGACACTATA
  • 3′ sequencing primer GCATTCTAGTTGTGGTTTGTCCAAACTCATC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Grx1-roCherry was a gift from Dmitry Bilan (Addgene plasmid # 141068 ; http://n2t.net/addgene:141068 ; RRID:Addgene_141068)

  • For your References section:

    Red fluorescent redox-sensitive biosensor Grx1-roCherry. Shokhina AG, Kostyuk AI, Ermakova YG, Panova AS, Staroverov DB, Egorov ES, Baranov MS, van Belle GJ, Katschinski DM, Belousov VV, Bilan DS. Redox Biol. 2019 Feb;21:101071. doi: 10.1016/j.redox.2018.101071. Epub 2018 Dec 7. 10.1016/j.redox.2018.101071 PubMed 30576927
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