pdUA-lacIN
(Plasmid #141079)

• Purpose: (Empty Backbone) Plasmid vector for testing DNA binding proteins by the I-Block assay.
• Depositing Lab: Antal Kiss
• Publication: Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28. doi: 10.1093/nar/gkaa014.

Backbone

• Vector backbone: Derived from pUA66
• Backbone manufacturer: pUA66 was described in Zaslaver et al. (2006) Nat. Methods 3: 623-628.
• Backbone size (bp): 4849
• Modifications to backbone: The Escherichia coli lacI gene carrying a modified promoter (lacIN) was cloned into pUA66. The gene of the green fluorescent protein and an EcoRI site originally present in pUA66 were deleted.
• Vector type: Bacterial Expression ; Vector for the I-Block assay designed to study DNA-protein interaction in E. coli.
• Promoter: Escherichia coli lacIN promoter

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ER1821dellacI
• Growth instructions: The strain ER1821dellacI can be grown at 37°C. For the I-Block assay, growth at 30°C is recommended.
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid pUA66 was described in Zaslaver et al. (2006) Nat. Methods 3: 623-628.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.