E. coli ER1821ΔlacI
(Bacterial strain #141407)

• Purpose: LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively
• Depositing Lab: Antal Kiss
• Publication: Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28. doi: 10.1093/nar/gkaa014.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 30°C
• Growth Strain(s): E. coli ER1821ΔlacI
• Growth instructions: The strain ER1821ΔlacI can be grown at 37°C. For the I-Block assay growth at 30°C is recommended.
• Copy number: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

λ- F- glnX44 e14- (McrA-) rfbD1 endA1 thi-1 Δ(yjiT-opgB)114::IS10 (EcoKI R- M- McrBC- Mrr-) + rpoS393(am) creC510 lrhA::IS3 ydeN::IS10 ΔlacI

Verification of lacI deletion:
PCR reaction on genomic DNA using AK362 and AK365 primers produces a 1936 bp fragment
AK 362 5’-CAATACCAATCGCACGCGG
AK 365 5’-CGAGACGTCACGGAAAATGCC

Phenotype: Constitutive β-galactosidase synthesis

This strain was derived from the precursor strain, E. coli ER1821, which is described in Jobling et al. (2016) Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems. Genome Announc 4(4):e00763-16. https://www.ncbi.nlm.nih.gov/pubmed/27516504