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(Plasmid #141080)


Item Catalog # Description Quantity Price (USD)
Plasmid 141080 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 9968
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible zCas9(H840A)-OsM-MLV-RT

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Tags / Fusion Proteins
    • 3x FLAG, NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ166-OsPE2 was a gift from Yiping Qi (Addgene plasmid # 141080 ; ; RRID:Addgene_141080)
  • For your References section:

    Plant Prime Editors Enable Precise Gene Editing in Rice Cells. Tang X, Sretenovic S, Ren Q, Jia X, Li M, Fan T, Yin D, Xiang S, Guo Y, Liu L, Zheng X, Qi Y, Zhang Y. Mol Plant. 2020 Mar 25. pii: S1674-2052(20)30072-1. doi: 10.1016/j.molp.2020.03.010. 10.1016/j.molp.2020.03.010 PubMed 32222487