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Antares-SEPLuc
(Plasmid #141086)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141086 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 4606
  • Total vector size (bp) 9178
  • Modifications to backbone
    Neomycin deleted
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Antares-T2A-puromycin-EMCV IRES-SEPLuc
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    4572
  • Mutation
    Deletion of amino acids 66 to 91 on the Luciferase region (Please see depositor comments)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7 forward (TTAATACGACTCACTATAGGG)
  • 3′ sequencing primer BGH reverse (TAGAAGGCACAGTCGAGG)
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deletion in the luciferase does not affect the BRET efficiency of Antares

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Antares-SEPLuc was a gift from Jeak Ling Ding (Addgene plasmid # 141086 ; http://n2t.net/addgene:141086 ; RRID:Addgene_141086)
  • For your References section:

    pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis. Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL. Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. 10.3389/fbioe.2020.00412 PubMed 32457886