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pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis.
Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
141082SEP-Nanoluc-cyOFP1prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis
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141084SEPLuc-E2A-cyOFP1precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis
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141085SEPLuc-IRES-Antaresprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
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141086Antares-SEPLucprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis
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141087Antares-SEPLuc IRESv24precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES
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141088Antares-SEPLuc C1A4Eprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
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141089pHLuc (Antares-SEPLuc C1A4E+6)final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis
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141091pHLuc ControlpH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP
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Recombinant Antibodies from Article