pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis.
Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. PubMed Article
Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141082 | SEP-Nanoluc-cyOFP1 | prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis |
| 141084 | SEPLuc-E2A-cyOFP1 | precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis |
| 141085 | SEPLuc-IRES-Antares | precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis |
| 141086 | Antares-SEPLuc | precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis |
| 141087 | Antares-SEPLuc IRESv24 | precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES |
| 141088 | Antares-SEPLuc C1A4E | precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc |
| 141089 | pHLuc (Antares-SEPLuc C1A4E+6) | final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis |
| 141091 | pHLuc Control | pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP |
| 141102 | SEPLuc | bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis |