Double UP mNeon to mScarlet FRT
(Plasmid #141111)

• Purpose: (Empty Backbone) Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.
• Depositing Lab: Erik Dent
• Publication: Taylor et al Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020.

Backbone

• Vector backbone: pCAX
• Backbone size (bp): 6734
• Modifications to backbone: Removed Not1 site in mScarlet
• Vector type: Mammalian Expression
• Promoter: CAG

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAGCTCCTGGGCAACGTGC
• 3′ sequencing primer: m13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Intent is to introduce low doses of pCAG FlpE to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.

Please visit https://www.biorxiv.org/content/10.1101/571323v1 for bioRxiv preprint.