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pcDNA4/TO/GFP-TTP
(Plasmid #141190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141190 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 141190-D50 50 μg of DNA in Tris buffer $495
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DNA 141190-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4/TO/GFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TTP
  • Alt name
    mRNA decay activator protein ZFP36
  • Alt name
    Tristetraprolin
  • Alt name
    Zinc finger protein 36
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    ZFP36 (a.k.a. G0S24, GOS24, NUP475, RNF162A, TIS11, TTP, zfp-36)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO/GFP-TTP was a gift from Simon Bekker-Jensen (Addgene plasmid # 141190 ; http://n2t.net/addgene:141190 ; RRID:Addgene_141190)

  • For your References section:

    GIGYF1/2-Driven Cooperation between ZNF598 and TTP in Posttranscriptional Regulation of Inflammatory Signaling. Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S. Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 10.1016/j.celrep.2019.03.006. 10.1016/j.celrep.2019.03.006 PubMed 30917308
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