XClone_Cas9_KRAB_T2A_BFP
(Plasmid #141208)

• Purpose: Tunable and temporal expression control of dCas9
• Depositing Lab: Rory Johnson
• Publication: Tunable and temporal expression control of Cas9 (unpublished)

Backbone

• Vector backbone: XLone-GFP
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Alt name: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
• Species: Streptococcus pyogenes

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • Annotated sequence_XClone_Cas9_KRAB_T2A_BFP
• A portion of this plasmid was derived from a plasmid made by: Xiaojun Lian (Addgene plasmid # 96930)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

derivatives of Addgene plasmid # 96930