GB1-PRD(800-868)
(Plasmid
#141345)
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PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-11a
- Backbone size w/o insert (bp) 5643
- Total vector size (bp) 6130
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGB1His6-TEV-ALIX(800-868)
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SpeciesH. sapiens (human)
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Insert Size (bp)487
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Tag
/ Fusion Protein
- GB1-His6-TEV (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GB1-PRD(800-868) was a gift from Lalit Deshmukh (Addgene plasmid # 141345 ; http://n2t.net/addgene:141345 ; RRID:Addgene_141345) -
For your References section:
Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids. Elias RD, Ma W, Ghirlando R, Schwieters CD, Reddy VS, Deshmukh L. Proc Natl Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 10.1073/pnas.2010635117. 10.1073/pnas.2010635117 PubMed 32917811
