A3RXB7
(Plasmid
#177694)
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Purposeexpresses a N-acetyl hexosamine oxidase in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a
- Backbone size w/o insert (bp) 5361
- Total vector size (bp) 6912
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN-acetylhexosamine oxidase
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Alt nameHexNACO
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SpeciesRalstonia solanacearum UW551
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Insert Size (bp)1551
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GenBank IDRRSL_02030
- Promoter T7
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Tag
/ Fusion Protein
- 10xHis (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTwistBioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
A3RXB7 was a gift from Martin Engqvist (Addgene plasmid # 177694 ; http://n2t.net/addgene:177694 ; RRID:Addgene_177694) -
For your References section:
Discovery of Two Novel Oxidases Using a High-Throughput Activity Screen. Rembeza E, Boverio A, Fraaije MW, Engqvist MKM. Chembiochem. 2022 Jan 19;23(2):e202100510. doi: 10.1002/cbic.202100510. Epub 2021 Nov 18. 10.1002/cbic.202100510 PubMed 34709726
