MBP-CRY1-PHR
(Plasmid
#26035)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMAL
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCRY1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1500
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Entrez GeneCRY1 (a.k.a. AT4G08920, ATCRY1, BLU1, BLUE LIGHT UNINHIBITED 1, CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR, ELONGATED HYPOCOTYL 4, HY4, OOP2, OUT OF PHASE 2, T3H13.14, T3H13_14, cryptochrome 1)
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Tag
/ Fusion Protein
- MBP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-CRY1-PHR was a gift from Johann Deisenhofer (Addgene plasmid # 26035 ; http://n2t.net/addgene:26035 ; RRID:Addgene_26035) -
For your References section:
Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana. Brautigam CA, Smith BS, Ma Z, Palnitkar M, Tomchick DR, Machius M, Deisenhofer J. Proc Natl Acad Sci U S A. 2004 Aug 17. 101(33):12142-7. 10.1073/pnas.0404851101 PubMed 15299148