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pU6-STOP-shA1
(Plasmid #14422)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14422 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS-cas2
  • Backbone manufacturer
    Rene Maehr
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, RNAi, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    633

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer M13 forward or M13 reverse
  • 3′ sequencing primer M13 forward or M13 reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

inducible shRNA expression vector. shRNA coding region can be replaced as follows: design ~80 bp oligos with 5’ BamHI site followed by mutant loxP, where the last 5 bp are replaced by the first 5 bp of shRNA sequence, followed by the remaining shRNA sequence, hairpin and as shRNA sequence, T stretch and SalI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-STOP-shA1 was a gift from Klaus Rajewsky (Addgene plasmid # 14422 ; http://n2t.net/addgene:14422 ; RRID:Addgene_14422)
  • For your References section:

    Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages. Oberdoerffer P, Kanellopoulou C, Heissmeyer V, Paeper C, Borowski C, Aifantis I, Rao A, Rajewsky K. Mol Cell Biol. 2005 May . 25(10):3896-905. 10.1128/MCB.25.10.3896-3905.2005 PubMed 15870264