pAd-Track Flag GCN5 Y621A/P622A
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14425||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
Vector typeMammalian Expression, Adenoviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGCN5 Y621A/P622A
SpeciesH. sapiens (human)
MutationY621A, P622A. Catalytically inactive acetyltransferase mutant
Entrez GeneKAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
/ Fusion Protein
- flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
The catalytically inactive acetyltransferase mutant GCN5 (Y621A/P622A) (GCN5m) was generated by site-directed mutagenesis and verified by DNA sequencing.
pAdTrack can be recombined with pAdEasy to make adenoviral-GCN5m.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.
Please note that Addgene quality control sequencing confirmed 2 additional mutations (E189G and K195N) which do not alter the plasmid's function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Track Flag GCN5 Y621A/P622A was a gift from Pere Puigserver (Addgene plasmid # 14425 ; http://n2t.net/addgene:14425 ; RRID:Addgene_14425)
For your References section:GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P. Cell Metab. 2006 Jun . 3(6):429-38. 10.1016/j.cmet.2006.04.013 PubMed 16753578