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pAd-Track Flag GCN5 Y621A/P622A
(Plasmid #14425)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14425 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAdTrack
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN5 Y621A/P622A
  • Alt name
    GCN5
  • Species
    H. sapiens (human)
  • Mutation
    Y621A, P622A. Catalytically inactive acetyltransferase mutant
  • Entrez Gene
    KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The catalytically inactive acetyltransferase mutant GCN5 (Y621A/P622A) (GCN5m) was generated by site-directed mutagenesis and verified by DNA sequencing.

pAdTrack can be recombined with pAdEasy to make adenoviral-GCN5m.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.

Please note that Addgene quality control sequencing confirmed 2 additional mutations (E189G and K195N) which do not alter the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track Flag GCN5 Y621A/P622A was a gift from Pere Puigserver (Addgene plasmid # 14425 ; http://n2t.net/addgene:14425 ; RRID:Addgene_14425)
  • For your References section:

    GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P. Cell Metab. 2006 Jun . 3(6):429-38. 10.1016/j.cmet.2006.04.013 PubMed 16753578